I HAEMOGRAM LAB

• Haemogram including Haemoglobin, TLC, DLC, Platelet count, MCV, MCH, RDW, Haematocrit, peripheral smear, reticulocyte count.
• Bone marrow morphology from bone marrow aspirate and bone marrow touch smear-Giemsa stain
• Bone marrow biopsy haematoxylin eosin stain
• Cytochemistry & immunophenotyping

II ANEMIA LAB

• Haemoglobin A2 and F estimation
• High performance liquid chromatography (HPLC) for detection of abnormal haemoglobins & their quantitation including HbF and A2
• Hb electrophoriesis for HbF & A2 and Haemoglobinopathy
• Sickling test
• Serum iron studies- S. iron, TIBC, % saturation
• Coomb’s test both direct and indirect manual and by gel technology
• Ham’s test and sucrose lysis test for PNH
• Heinz bodies /HbH inclusion
• G6PD screening test
• Osmotic fragility test
• Plasma haemoglobin
• Urine haemosiderin
• Serum protein electrophoresis for plasma cell dyscrasia
• Cryoglobulin

III COAGULATIO

• Bleeding time
• Prothrombin time
• Partial thromboplastin time with Kaolin
• Thrombin time
• Fibrinogen
• Euglobulin clot lysis time
• Clot solubility test
• D-Dimer
• Factor assay for – Factor V, VIII, IX, X and XIII
• Inhibitor studies for Factor V, VIII, IX, X and XIII
• PF3 availability test with ADP
• Total PF3
• Platelet function test
• INR for monitoring of oral anticoagulant therapy
• Lupus anticoagulant
• thrombophilia work up [ AT II, Protein C, Protein S, APC-R] Proglob C
• Ristocetin Co-F
• VWD antigen
• Beta 2 glycoprotein

MOLECULAR TESTS

• Thrombophilic mutations
  • Prothrombin P20210 G®A mutation
  • MTHFR C6 77T gene polymorphism
  • Factor V leiden mutation
• Haemophilia
  • Intron 22 inversion
  • Intron 1 inversion
  • Carrier detection and prenatal diagnosis by linkage analysis
• Glanzmann’s Thrombasthenia
  • GP IIb//IIIa western blotting
  • GP IIb//IIIa flow cytometry
• VWD
  • Multimeric analysis by western blotting
  • Carrier detection by linkage analysis
• Thalassemia
  • Alpha thalassemia mutation- 3.7 and 4.2 deletions
• Leukemia
  • RT-PCR for
    • CML (BCR-ABL) Philadelphia
    • APML t(15;17)
    • AML t(8;21), Inv 16
    • ALL t(9;22), t(1;19), t(12;21), t (4;11)

CYTOCHEMISTRY

• Sudan black (SB)
• Myeloperoxidase (MPO)
• Leukemia alkaline phosphatase (LAP)
• PAS,
• NSE and NSE-F (Non specific esterase)
• Acid phosphatase
• Double esterase
• Real Time PCR

IMMUNOPHENOTYP

• Lab provides immunophenotyping for acute and chronic leukemias, PNH, Platelet flowcytometry.