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Section of Parasitology, Department of Microbiology, is the first dedicated laboratory that provides investigations for diseases caused by parasites. The laboratory has received NABL accreditation. Investigations are available for the diseases caused by various medically important protozoa, helminths and arthropod agents. Many of these diseases are of immense public health importance and include malaria, visceral leishmaniasis, lymphatic filariasis, and intestinal parasitic infections. Microscopic techniques such as direct microscopic examination of unstained preparations of clinical specimens, examination of stained smears and special investigations such as Quantitative Buffy Coat (QBC) assay and immunochromatography test (ICT) are routinely used for some of the diseases. Rapid diagnostic tests are used for diagnosis of different parasitic infections of public health importance and includes antigen detection for malaria (pLDH & HRP-II) and lymphatic filariasis, and antibody detection assays such as, rK 39 test for visceral leishmaniasis, Enzyme Linked Immuno-Sorbent Assay (ELISA) for toxoplasmosis, amoebic liver abscess, cystic hydatid disease, strongyloidiasis, toxocariasis, etc. are being carried out. The section also conducts molecular diagnostic investigations including Multiplex PCR assay for human malaria, and PCR assays for Pneumocystis jiroveci, Microsporidia & free living amoeba.

S.no.Test NameSample
1BLOOD (CITRATE) FOR EXAMINATION FOR MICROFILARIA
Acridine orange staining for microfilaria (microscopy)
Giemsa staining for microfilaria (microscopy)
Quantitative buffy coat assay (microscopy)
Direct & Concentration mount for microfilaria (microscopy)
Filarial Antigen detection test
BLOOD (CITRATE)
2BLOOD (EDTA) FOR MALARIA WORKUP
Acridine orange staining for Malaria parasite (microscopy)
Giemsa staining for Malaria parasite (microscopy)
Quantitative buffy coat assay for Malaria parasite (microscopy)
Malaria parasite antigen detection test
BLOOD (EDTA)
3MULTIPLEX PCR ASSAY FOR HUMAN MALARIABLOOD (EDTA)
4BLOOD (EDTA) FOR MICROSCOPY FOR PARASITES (OTHER THAN MALARIA)BLOOD (EDTA)
5BONE MARROW FOR MICROSCOPIC EXAMINATION FOR LD BODIES (KALA AZAR)
Acridine orange staining for LD bodies
Giemsa staining for LD bodies
BONE MARROW (EDTA/SMEAR)
6BONE MARROW FOR MICROSCOPY FOR PARASITES (OTHER THAN KALA AZAR)BONE MARROW (EDTA/SMEAR)
7BRONCHO-ALVEOLAR LAVAGE FOR MICROSCOPIC EXAMINATION FOR Pneumocystis jiroveci (PCP)BRONCHO-ALVEOLAR LAVAGE FLUID
8BRONCHO-ALVEOLAR LAVAGE - PCR FOR Pneumocystis jiroveci (PCP)BRONCHO-ALVEOLAR LAVAGE FLUID
9BRONCHO-ALVEOLAR LAVAGE FOR MICROSCOPY FOR PARASITES (OTHER THAN Pneumocystis jiroveci/PCP)BRONCHO-ALVEOLAR LAVAGE FLUID
10C.S.F FOR DIRECT MICROSCOPY FOR FREE LIVING AMOEBAEC.S.F
11C.S.F FOR MICROSCOPY FOR PARASITES (OTHER THAN FREE LIVING AMOEBAE)C.S.F
12DUODENAL ASPIRATE FOR MICROSCOPY FOR PARASITESDUODENAL ASPIRATE
13LIVER ABSCESS ASPIRATE FOR MICROSCOPY FOR AMOEBIASISLIVER ABSCESS ASPIRATE
14LIVER ABSCESS ASPIRATE FOR MICROSCOPY FOR PARASITES (OTHER THAN AMOEBIC LIVER ABSCESS)LIVER ABSCESS ASPIRATE
15LYMPH NODE ASPIRATE FOR MICROSCOPIC EXAMINATION FOR LD BODIES
Acridine orange staining for LD bodies
Giemsa staining for LD bodies
LYMPH NODE ASPIRATE
16LYMPH NODE ASPIRATE FOR MICROSCOPY FOR PARASITES (OTHER THAN LEISHMANIASIS)LYMPH NODE ASPIRATE
17PROSTATIC SECRETION FOR MICROSCOPY FOR Trichomonas vaginalisPROSTATIC SECRETION
18SERUM FOR ELISA FOR AMOEBIASISSERUM
19SERUM FOR ELISA FOR CYSTIC ECHINOCOCCOSISSERUM
20SERUM FOR ELISA FOR TOXOPLASMOSISSERUM
21SERUM FOR ANTI-Strongyloides IgG ELISASERUM
22SERUM FOR ANTI-Toxocara canis IgG ELISASERUM
23SERUM FOR ANTI-Taenia solium IgG ELISASERUM
24SERUM FOR rK 39 ANTIBODY DETECTION FOR LEISHMANIASISSERUM
25SIGMOIDOSCOPY ASPIRATE FOR MICROSCOPY FOR PARASITESSIGMOIDOSCOPY ASPIRATE
26SPLENIC ASPIRATE FOR MICROSCOPY FOR LD BODIES (KALA AZAR)SPLENIC ASPIRATE
27SPUTUM FOR MICROSCOPY FOR PARASITES (OTHER THAN Pneumocystis jiroveci/PCP)SPUTUM
28STOOL EXAMINATION FOR PARASITES (TROPHOZOITES/CYSTS/OOCYSTS OF COCCIDIA/OVA/LARVAE)
Stool direct/concentration mounts microscopy
Modified acid-fast staining for coccidian parasites (Cryptosporidium spp./Cyclospora cayetanensis/Cystoisospora belli)
STOOL
29URETHRAL DISCHARGE FOR MICROSCOPY FOR Trichomonas vaginalisURETHRAL DISCHARGE
30URINE FOR MICROSCOPY FOR PARASITESURINE
31IDENTIFICATION OF MEDICALLY IMPORTANT ARTHROPODSARTHROPOD SPECIMEN
32IDENTIFICATION OF PARASITIC SEGMENTS/WORMSSEGMENT/WORM SPECIMEN
33SPUTUM FOR MICROSCOPIC EXAMINATION FOR Pneumocystis jiroveci (PCP)
GMS staining for Pneumocystis jiroveci
SPUTUM
34SPUTUM FOR PCR FOR Pneumocystis jiroveci (PCP)SPUTUM
35STOOL FOR in vitro CULTURE FOR HOOKWORMSTOOL
36STOOL FOR MICROSCOPIC EXAMINATION FOR MICROSPORIDIA
Calcofluor white staining for microsporidia
Modified trichrome staining for microsporidia
STOOL